Objectives: Nurses, as the largest group providing health care services, play a critical role in the continuity of care and respond to services commensurate with their competence. Professional competence in nursing is a complex and ambiguous concept and needs the redefi nition of competence and development of its defi nition and concept. In this study, we aimed to conceptually analyze professional competence in nursing. Methods: In this study, a hybrid concept analysis was used. In the literature review phase, the concept of ‘ competence’ was investigated through valid databases limited to 2000-2018 and the participants’ experiences also appeared in the fi eldwork phase. In the fi eldwork phase, the participants revealed some aspects of professional competence in nursing, which were not well-addressed in the literature. Finally, the comprehensive defi nition of professional competence in nursing was presented after integrating the fi ndings obtained in these two phases. Results: According to the fi ndings, professional competence in nursing is a combined, complex, multidimensional, and contextdependent concept, and consists of diff erent domains. Based on the analysis results of the fi eldwork phase, this concept was classifi ed into 16 domains and the participants largely contributed to the provision of a comprehensive defi nition based on the defi nitions of professional competence presented in the literature. Conclusions: Based on the domains of professional competence in nursing obtained from this study, an appropriate instrument can be developed to assess professional competence in nursing for students and nurses.